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portrait of faculty member Neelamegham
  

Sriram Neelamegham

Professor

 

906 Furnas Hall
(716) 645-2911 ext. 2220
Fax: (716) 645-3822
neel@eng.buffalo.edu
Short bio Research Publications Teaching Home page

Chapters in Books

  • Beauharnois, M.B., Neelamegham, S. and Matta, K.L., "Quantitative measurement of selectin-ligand interactions: Assays to identify a sweet pill in a library of carbohydrates ", Glycobiology Protocols- Methods Mol Biol. 2005.
  • Zhang, Y. and Neelamegham, S., "Blood Cell, Counter", Encyclopedia of Medical Devices and Instrumentation. Second Ed. 2005.
  • Neelamegham, S. and Matta, K.L., "Liposomes bearing carbohydrates: Ligands for selectins", 1999:175-91, Liposome- Methods Mol Biol. 2002.

Patents

  • "Recovery of liquid from absorbent packaging material", Alexandridis, P., and Neelamegham, S., U.S. patent 6,589,797, 2003 [Licensed to Technicor, Inc. Amherst, NY].
  • “Novel family of selectin antagonists­­­­­”, Neelamegham, S., and Matta, K.L., U.S. provisional patent 60/670,163, 2005.

Selected Refereed Journal Publications

  • Singh, I., Shankaran, H., Beauharnois, M.E., Xiao, Z., alexandridis, P., Neelamegham, S., "Solution structure of human von Willebrand factor studied using small angle neutron scattering," J. Biol. Chem., 281 (50): 38266-75, (2006).
  • Chandrasekaran, E.V., Xue, J., Neelamegham, S., Matta, K.L., "The pattern of glycosyl- and sulfotransferase activities in cancer cell lines:  A predictor of individual cancer-associated distinct carbohydrate structures for the structural identification of signature glycans," Carbohydr. Res., 341:  983-994, (2006).
  • Xiao, Z., Goldsmith, H.L., McIntosh, F.A., Shankaran, H., Neelamegham, S., “P-selectin PSGL-1 bonds in neutrophil-platelet adhesion: Shear threshold and integrin-independent cell adhesion”, Biophys. J., 90(6): 2221-34, 2005.
  • Beauharnois, M.E., Lindquist, K.C., Marathe, D., Vanderslice, P., Xia, J., Matta, K.L., Neelamegham, S. “Affinity and Kinetics of Sialyl Lewis-X and Core-2 based Oligosaccharides Binding to L- and P-selectin ”, Biochemistry, 44(27): 9507-19, 2005.
  • Chandrasekaran, E.V. Xue, J., Xia, J., Chawda, R., Piskorz, C., Locke, R.D., Neelamegham, S., Matta, K.L. “Specificity analysis of sialyltransferases towards mucin core-2, Globo and related structures: Identification of the sialylation-sequence and the effects of sulfate, fucose, methyl and fluoro substituents of the carbohydrate chain in the biosynthesis of selectin and siglec ligands, and novel sialylation by cloned α2,3(O)sialyltransferase ”, Biochemistry, 44(47), 15619-35, 2005.

  • Liu, G., Swihart, M.T., Neelamegham, S., "Sensitivity, principal component and flux analysis applied to signal transduction: The case of epidermal growth factor mediated signaling", Bioinformatics, 21(7):1194-202, 2005.

  • McDonough DB, McIntosh FA, Spanos C, Neelamegham S, Goldsmith HL, Simon SI., "Cooperativity between selectins and beta2-integrins define neutrophil capture and stable adhesion in shear flow," Ann Biomed Eng., 32(9):1179-92, 2004.

  • Neelamegham, S. "Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion", Cell Communication and Adhesion, 11(1): 35-50, 2004.
  • Chandrasekaran EV, Lakhaman SS, Chawda R, Piskorz CF, Neelamegham S, Matta KL. "Identification of physiologically relevant substrates for cloned Gal: 3-O-sulfotransferases (Gal3STs): distinct high affinity of Gal3ST-2 and LS180 sulfotransferase for the globo H backbone, Gal3ST-3 for N-glycan multiterminal Galbeta1, 4GlcNAcbeta units and 6-sulfoGalbeta1, 4GlcNAcbeta, and Gal3ST-4 for the mucin core-2 trisaccharide", J. Biol. Chem., 279(11): 10032-41, 2004.
  • Shankaran H., and Neelamegham, S. "Hydrodynamic forces applied on intercellular bonds, soluble molecules and cell surface receptors", Biophys J.,  86(1): 576-588, 2004.
  • Zhang, Y., and Neelamegham, S., "An analysis tool to quantify the efficiency of cell tethering and firm-adhesion in the parallel-plate flow chamber", Journal of Immunological Methods,  278(1-2): 305-317, 2003.
  • Zhang, Y., and Neelamegham, S.,”Hydrodynamic Recruitment of Rolling Leukocytes In Vitro ”, Biophysical J., 84:4183 (2003).
  • Shankaran H, Alexandridis P, Neelamegham S., "Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-association of von Willebrand factor in suspension", Blood, 101(7): 2637-45, 2003.
  • Zhang Y., Neelamegham, S., "PPLATE: A program for analysis of parallel plate flow chamber experimental data", Journal of Immunological Methods, 278(1-2): 319-321, 2003.
  • Zhang, Y., and Neelamegham, S., "Estimating the efficiency of cell capture and arrest in flow chambers: Study of neutrophil binding via E-selectin and ICAM-1", Biophys. J., 83(4): 1934-52, 2002.
  • Shankaran H., and Neelamegham, S. "Effect of Secondary Flow on Biological Experiments in the Cone-Plate Viscometer: Methods for estimating Collision Frequency, Wall Shear Stress and inter-particle Interactions", Biorheology, 38(4): 275-304, 2001.
  • Ahmed F., Alexandridis P., Shankaran, H., Neelamegham S., "The Ability of Poloxamers to Inhibit Platelet Aggregation Depends on Their Physicochemical Properties", Thrombosis and Homeostasis, 86(6),1532-9, 2001.
  • Shankaran, H. and Neelamegham, S., "Non-Linear Flow Affects Hydrodynamic Forces and Neutrophil Adhesion Rates in Cone-Plate Viscometers" Biophysical J., 80(6): 2631-2648, 2001.
  • Ahmed, F. Alexandridis, P., Neelamegham, S., "Synthesis and Application of Fluorescein Labeled Pluronic Block Copolymers to Study Polymer-Surface Interactions", Langmuir, 17: 537-546, 2001.
  • Neelamegham S., Taylor A.D., Shankaran, H., Smith, C.W., Simon, S.I., "Shear and Time-Dependent Changes in Mac-1, LFA-1, and ICAM-3 Binding Regulate Neutrophil Homotypic Adhesion", Journal of Immunology, 164(7): 3798-3805, 2000.
  • Hentzen E.R., Neelamegham S., Kansas, G., McIntire, L.V., Smith, C.W., Simon, S.I., "Sequential binding of CD11a/CD18 and CD11b/CD18 define neutrophil capture and stable adhesion to ICAM-1", Blood, 95(3): 911-920, 2000.
  • Hellums, J.D., Taylor, A.D., Neelamegham, S., Simon, S.I., "Rheology and molecular events in neutrophil aggregation", Journal of the Japanese Society of Biorheology, 12(1): 12-35, 1998.
  • Neelamegham, S., Taylor A.D., Burns, A.R., Smith, C.W., Simon, S.I., "Hydrodynamic shear reveals distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to ICAM-1", Blood, 92(5): 1626-1638, 1998.
  • *Konstantopolous, K., *Neelamegham, S.,  Burns, A.R., Hentzen, E., Kansas, G.S., Snapp, K.R., Berg, E.L., Hellums, J.D., Smith, C.W., McIntire, L.V., Simon, S.I., "Venous levels of shear induces neutrophil-platelet and neutrophil aggregation in blood via P-selectin and b2-integrin", Circulation, 98: 873-882, 1998. * Both authors are joint first authors.
  • Simon, S.I. Neelamegham S., Taylor A.D., Smith C.W., "The multistep process of homotypic neutrophil aggregation: a review of the molecules and affects of hydrodynamics", Cell Adhesion and Communication, 6(2-3): 263-76, 1998.
  • Lynam E., Sklar L.A., Taylor A.D., Neelamegham S., Edwards, B.S., Smith, C.W., Simon, S.I., "Contributions of Mac-1 and LFA-1 in neutrophil adhesion to an ICAM-1 transfectant under hydrodynamic shear", Journal of Leukocyte Biology, 64(5): 622-30, 1998.
  • Neelamegham, S., Munn, L.L., Zygourakis, K., "A mathematical model for the kinetics of homotypic cellular aggregation under static conditions", Biophysical Journal, 72: 51-64, 1997.
  • Neelamegham, S., Zygourakis, K., "A quantitative assay for intercellular aggregation", The Annals of Biomedical Engineering, 25 (1): 180-189, 1997.
  • Neelamegham, S., Taylor, A.D., Hellums J.D., Dembo M., Smith C.W., Simon, S.I., "Modeling the reversible kinetics of neutrophil aggregation under hydrodynamic shear", Biophysical Journal, 72: 1527-1540, 1997.
  • McIntyre, B.W., Woodside, D.G., Caruso, D.A., Wooten, D.K., Simon, S.I., Neelamegham, S., Revelle, J.K., Vanderslice, P., "Regulation of human lymphocyte coactivation with an a4 integrin antagonist peptide", Journal of Immunology, 158: 4180-4186, 1997.
  • Tsang, Y.T.M., Neelamegham, S., Hu, Y., Burns, A.R., Berg, E.L. , Smith, C.W., Simon, S.I., "Synergy between L-selectin signaling and chemotactic activation during neutrophil adhesion and transmigration", Journal of Immunology, 159: 4566-4579, 1997.
  • Neelamegham, S., Simon, S.I., McIntyre, B.W., Zygourakis, K., "Induction of homotypic lymphocyte aggregation: evidence for a novel activation state of the b1 Integrin.", Journal of Leukocyte Biology, 59: 872-882 , 1996.
  • Taylor, A.D., Neelamegham, S., Hellums, J.D., Smith, C.W., Simon, S.I. "Molecular dynamics of the transition from L-selectin to beta 2-Integrin dependent neutrophil adhesion under defined hydrodynamic shear", Biophysical Journal, 71: 3488-3500, 1996.

(Please see investigator homepage for links to individual manuscript abstracts and supplemental material).

Last Updated: October 2007

303 Furnas Hall • Buffalo, New York 14260-4200 • (716) 645-2911 • Fax: (716) 645-3822